Reconstitution of the partially purified renal phosphate (Pi) transporter
نویسندگان
چکیده
منابع مشابه
The Preparation of Partially Purified
The treatment of tuberculous meningitis is frequently hindered by the formation of exudate within the cerebrospinal spaces. This exudate may obstruct the cerebrospinal circulation and give rise to the complications of subtentorial, basal cistern, and spinal block. Furthermore, the presence of exudate predisposes to the occurrence of tuberculous arteritis, which is responsible for the cerebral a...
متن کاملReconstitution of neutral amino acid transport from partially purified membrane components from Ehrlich ascites tumor cells.
Solubilized protein fractions have been obtained from plasma membranes of Ehrlich ascites cells either by extraction with 0.5% Triton X-100 or by extraction with 2% cholate. Partial purification of the solubilized protein fraction has been obtained by utilizing a combination of ammonium sulfate precipitation and column chromatography. Leucine-binding activity has been detected in the Triton X-1...
متن کاملProperties of electrogenic Pi transport by a human renal brush border Na+/Pi transporter.
Inorganic phosphate (Pi) induced an inward current (IP) in Xenopus oocytes expressing the human renal Na+/Pi cotransporter NaPi-3. At 100mM Na+, Pi-transport was independent of the holding potential and resulted in an apparent Km of 0.08 mM; lowering the Na+ concentration to 50 mM resulted in an increase of the apparent Km to 0.22 mM at -50 mV and to 0.31 mM at -90 mV. In contrast, the apparent...
متن کاملEffect of dietary components on renal inorganic phosphate (Pi) excretion induced by a Pi-depleted diet.
Dietary inorganic phosphate (Pi) is the most important factor in the regulation of renal Pi excretion. Recent studies suggest the presence of an enteric-renal signaling axis for dietary Pi as well as the existence of a mechanism by which the intestine detects changes in luminal Pi concentrations. The mechanisms of intestinal Pi sensing, however, are unknown. In the present study, we focused on ...
متن کاملFunctional expression, purification and reconstitution of the recombinant phosphate transporter Pho89 of Saccharomyces cerevisiae
The Saccharomyces cerevisiae high-affinity phosphate transporter Pho89 is a member of the inorganic phosphate (Pi) transporter (PiT) family, and shares significant homology with the type III Na(+)/Pi symporters, hPit1 and hPit2. Currently, detailed biochemical and biophysical analyses of Pho89 to better understand its transport mechanisms are limited, owing to the lack of purified Pho89 in an a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1986
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2350189